Got frameshift problems? In their article in mBio this week, authors from Michigan State University and Yonsei University in South Korea present a new tool for sequence analysis:
But what's a tool without some testing? With amplicon sequence data from defined communities for three different functional genes, FrameBot performed better than two other rapid frameshift correction tools, FragGeneScan and HMMFrame. Wang et al. also put all three tools to work on a very large dataset: 1.1 million nifH 454 amplicon sequences from 222 soil samples collected from sites in Alaska, Hawaii, Utah, and Florida. In general, FrameBot was more accurate than the other tools as long as a reference protein sequence with 80% or greater identity to a query was available, as was the case for virtually all nifH reads for the 4 sites.